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Structured Review

Proteintech anti bub1
Anti Bub1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+bub1/BUB1+Antibody/pmc12571864-80-27-29
Average 93 stars, based on 17 article reviews
anti bub1 - by Bioz Stars, 2026-09
93/100 stars

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Related Articles

Incubation:

Article Title: Exploring the carcinogenic potential of bisphenol A in lung adenocarcinoma: molecular mechanisms, key gene insights, and immune microenvironment impacts
Article Snippet: Equal amounts (30 μg) of protein were separated on 10% SDS–PAGE gels and transferred to PVDF membranes (Millipore, Billerica, MA, USA). .. Membranes were blocked with 5% non-fat milk in TBST for 1 h at room temperature, followed by overnight incubation at 4 °C with the following primary antibodies: anti-BUB1 (1:1000, Proteintech, Cat# 13330-1-AP), anti-BUB1B (1:1000, Proteintech, Cat# 11504-2-AP), anti-CCNA2 (1:1000, Proteintech, Cat# 18202-1-AP), anti-UBE2C (1:1000, Proteintech, Cat# 66087-1-Ig), anti-CDK1 (1:1000, Proteintech, Cat# 10762-1-AP), and anti-GAPDH (1:2000, Proteintech, Cat# 81640-5-RR). .. After washing, membranes were incubated with HRP-conjugated secondary antibodies (1:5000, Beyotime, Shanghai, China) for 1 h at room temperature.

Article Title: A New Method for Chromosomes Preparation by ATP-Competitive Inhibitor SP600125 via Enhancement of Endomitosis in Fish.
Article Snippet: Total proteins were separated by 12% SDS polyacrylamide gel electrophoresis and transferred to a polyvinylidene difluoride membrane (Millipore, Bedford, MA, USA). .. The membrane was blocked with 5% (w/v) non-fat milk in TBS containing 0.05% Tween20 (TBST) for 1 h at 37◦C and then incubated with primary antibodies, including anti-p53 (Abcam, USA), anti-MDM2 (Bioworld, USA), anti-p21 (Bioworld, USA), anti-Mad2L1 (Bioworld, USA), anti-CDC20 (Bioworld, USA), anti-BUB1 (Proteintech, USA), anti-Flag-tag (Proteintech, USA), and antiβ-ACTIN (Abcam, USA), overnight. ..

Article Title: A New Method for Chromosomes Preparation by ATP-Competitive Inhibitor SP600125 via Enhancement of Endomitosis in Fish
Article Snippet: Total proteins were separated by 12% SDS polyacrylamide gel electrophoresis and transferred to a polyvinylidene difluoride membrane (Millipore, Bedford, MA, USA). .. The membrane was blocked with 5% (w/v) non-fat milk in TBS containing 0.05% Tween-20 (TBST) for 1 h at 37°C and then incubated with primary antibodies, including anti-p53 (Abcam, USA), anti-MDM2 (Bioworld, USA), anti-p21 (Bioworld, USA), anti-Mad2L1 (Bioworld, USA), anti-CDC20 (Bioworld, USA), anti-BUB1 (Proteintech, USA), anti-Flag-tag (Proteintech, USA), and anti-β-ACTIN (Abcam, USA), overnight. ..

Membrane:

Article Title: A New Method for Chromosomes Preparation by ATP-Competitive Inhibitor SP600125 via Enhancement of Endomitosis in Fish.
Article Snippet: Total proteins were separated by 12% SDS polyacrylamide gel electrophoresis and transferred to a polyvinylidene difluoride membrane (Millipore, Bedford, MA, USA). .. The membrane was blocked with 5% (w/v) non-fat milk in TBS containing 0.05% Tween20 (TBST) for 1 h at 37◦C and then incubated with primary antibodies, including anti-p53 (Abcam, USA), anti-MDM2 (Bioworld, USA), anti-p21 (Bioworld, USA), anti-Mad2L1 (Bioworld, USA), anti-CDC20 (Bioworld, USA), anti-BUB1 (Proteintech, USA), anti-Flag-tag (Proteintech, USA), and antiβ-ACTIN (Abcam, USA), overnight. ..

Article Title: A New Method for Chromosomes Preparation by ATP-Competitive Inhibitor SP600125 via Enhancement of Endomitosis in Fish
Article Snippet: Total proteins were separated by 12% SDS polyacrylamide gel electrophoresis and transferred to a polyvinylidene difluoride membrane (Millipore, Bedford, MA, USA). .. The membrane was blocked with 5% (w/v) non-fat milk in TBS containing 0.05% Tween-20 (TBST) for 1 h at 37°C and then incubated with primary antibodies, including anti-p53 (Abcam, USA), anti-MDM2 (Bioworld, USA), anti-p21 (Bioworld, USA), anti-Mad2L1 (Bioworld, USA), anti-CDC20 (Bioworld, USA), anti-BUB1 (Proteintech, USA), anti-Flag-tag (Proteintech, USA), and anti-β-ACTIN (Abcam, USA), overnight. ..



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